ambient temperature (Proteintech)
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ambient temperature
Ambient Temperature, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 2559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ambient+temperature/ZO-1+Antibody/pm41903083-102-12-28
Average 97 stars, based on 2559 article reviews
Ambient Temperature, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 2559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ambient+temperature/ZO-1+Antibody/pm41903083-102-12-28
Average 97 stars, based on 2559 article reviews
ambient temperature - by Bioz Stars,
2026-10
97/100 stars
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Incubation:Article Title: Diagnostics and immunological function of CENPN in human tumors: from pan-cancer analysis to validation in breast cancer Article Snippet: Afterward, they were loaded into a 10% SDS-PAGE gel and deposited onto polyvinylidene fluoride (PVDF) membranes with a thickness of 0.45 mm (ISEQ00010, Millipore, city, USA). .. The PVDF membranes were obstructed for 1 h at Article Title: Chronic Excessive Exposure to Fluoride Impairs the Intestinal Mucosal Barrier to Cause Inflammatory Response and Microbiome Dysbiosis in Rats. Article Snippet: Extended author information available on the last page of the article Abstract Chronic excessive exposure to fluoride is known to cause dental fluorosis, skeletal fluorosis, and even disability.. We previously demonstrated that chronic fluorosis contributes to dysbiosis of the gut microbiota and metabolic disturbances in human; however, the underlying mechanisms remain unclear.. 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Immunofluorescence: CFs from passage 2 (P2) were fixed with 4% paraformaldehyde (PFA, Beijing Soleibao Technology Co., Ltd., China) for 20 minutes, washed thrice with PBS, permeabilized with 0.1% Triton-100 (Beijing Soleibao Technology Co., Ltd., China) for 8 minutes, sealed with 5% bovine serum albumin (BSA, Beijing Soleibao Technology Co., Ltd., China) for 60 minutes at Article Title: Ferroptosis’s Master Switch GPX4 emerges as universal biomarker for precision immunotherapy: a pan-cancer study with in vitro experiments validation Article Snippet: Cells were disrupted using chilled RIPA buffer from Beyotime (P0013, Shanghai, China), and samples were prepared in sodium dodecyl sulfate (SDS) buffer, separated by 12% SDS-PAGE, and then transferred onto a polyvinylidene fluoride (PVDF) membrane. .. Then, membranes were first blocked with 5% non-fat milk solution for 1 hour at Produced:Article Title: Diagnostics and immunological function of CENPN in human tumors: from pan-cancer analysis to validation in breast cancer Article Snippet: Afterward, they were loaded into a 10% SDS-PAGE gel and deposited onto polyvinylidene fluoride (PVDF) membranes with a thickness of 0.45 mm (ISEQ00010, Millipore, city, USA). .. The PVDF membranes were obstructed for 1 h at Membrane:Article Title: Chronic Excessive Exposure to Fluoride Impairs the Intestinal Mucosal Barrier to Cause Inflammatory Response and Microbiome Dysbiosis in Rats. Article Snippet: Extended author information available on the last page of the article Abstract Chronic excessive exposure to fluoride is known to cause dental fluorosis, skeletal fluorosis, and even disability.. We previously demonstrated that chronic fluorosis contributes to dysbiosis of the gut microbiota and metabolic disturbances in human; however, the underlying mechanisms remain unclear.. In this study, cellular and rat models of chronic fluorosis were utilized to investigate the mechanistic interactions between fluoride exposure and the intestinal barrier, mucosal immunity, and gut microbiome. Article Title: Methylprednisolone alleviates lung injury in sepsis by regulating miR-151-5p/USP38 pathway. Article Snippet: Background: Acute lung injury (ALI) is manifested by increased blood vessel permeability within the lungs and subsequent impairment of alveolar gas exchange.. Methylprednisolone (MP) is commonly used as a treatment for ALI to reduce inflammation, yet its molecular mechanism remains unclear.. This study aims to explore the underlying mechanisms of MP on ALI in a model induced by lipopolysaccharide (LPS). Blocking Assay:Article Title: Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration Article Snippet: .. Next, the products were transferred onto the PVDF membranes, followed by 2 h of blocking by 5% skimmed milk powder under Control:Article Title: Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration Article Snippet: .. Next, the products were transferred onto the PVDF membranes, followed by 2 h of blocking by 5% skimmed milk powder under Immunofluorescence:Article Title: Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury Article Snippet: Growth and morphological changes were observed with an inverted microscope (Olympus Corporation, Japan) at 40X and 100X magnification at 0H (immediately after differential adhesion for 90 minutes), 24H, 48H, and 72H. .. Immunofluorescence: CFs from passage 2 (P2) were fixed with 4% paraformaldehyde (PFA, Beijing Soleibao Technology Co., Ltd., China) for 20 minutes, washed thrice with PBS, permeabilized with 0.1% Triton-100 (Beijing Soleibao Technology Co., Ltd., China) for 8 minutes, sealed with 5% bovine serum albumin (BSA, Beijing Soleibao Technology Co., Ltd., China) for 60 minutes at Laser-Scanning Microscopy:Article Title: Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury Article Snippet: Growth and morphological changes were observed with an inverted microscope (Olympus Corporation, Japan) at 40X and 100X magnification at 0H (immediately after differential adhesion for 90 minutes), 24H, 48H, and 72H. .. 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Immunofluorescence: CFs from passage 2 (P2) were fixed with 4% paraformaldehyde (PFA, Beijing Soleibao Technology Co., Ltd., China) for 20 minutes, washed thrice with PBS, permeabilized with 0.1% Triton-100 (Beijing Soleibao Technology Co., Ltd., China) for 8 minutes, sealed with 5% bovine serum albumin (BSA, Beijing Soleibao Technology Co., Ltd., China) for 60 minutes at Saline:Article Title: Methylprednisolone alleviates lung injury in sepsis by regulating miR-151-5p/USP38 pathway. Article Snippet: Background: Acute lung injury (ALI) is manifested by increased blood vessel permeability within the lungs and subsequent impairment of alveolar gas exchange.. Methylprednisolone (MP) is commonly used as a treatment for ALI to reduce inflammation, yet its molecular mechanism remains unclear.. This study aims to explore the underlying mechanisms of MP on ALI in a model induced by lipopolysaccharide (LPS). |